DNA
Part:BBa_K3992002
Designed by: Ke ZHU Group: iGEM21_Shanghai_high-school (2021-10-12)
GS linker
GS linker
Profile
Name: GS linker
Base Pairs: 57bp
Origin: Synthetic
Properties: A linker to linked different proteins.
Usage and Biology
BBa_K3992002 is a part of GS linker. It can link different proteins to make them become a fusion protein. In our group, we use this part to link VP7 protein and LTB protein.
Construct Design
VP7-LTB is linked by GS linker (Figure 1).
Experimental approach
Polymerase Chain Reaction
A PCR verification was performed to confirm whether the sequence of synthesized VP7-LTB is cerrect. Agarose gel electrophoresis was used to assess the PCR’s result. According to the 15000 bp DNA marker, the PCR amplified DNA fragments possess the desired right size.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |